細胞名稱

兔角膜上皮細胞SIRC

貨物編碼

BFN60806664

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研           3類

培養體系

DMEM高糖+10%FBS+1%雙抗

培養溫度

37℃

二氧化碳濃度

5%

簡介

兔角膜上皮細胞SIRC，貼壁培養，不規則形態。該細胞源于JCRB。

序列突變

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注釋

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Characteristics: Can be used for isolation and growth of rubella virus.

Characteristics: Expresses a mix of epithelial and fibroblastic markers (PubMed=29653143).

Doubling time: ~31 hours (lot 11132012), ~1.5 days (lot 10272017) (JCRB).

STR信息

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參考文獻

PubMed=14331182; DOI=10.1126/science.149.3684.633

Leerhoy J.

Cytopathic effect of rubella virus in a rabbit-cornea cell line.

Science 149:633-634(1965)

PubMed=5918951; DOI=10.3181/00379727-122-31251

Phillips C.A., Melnick J.L., Burkhardt M.

Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells.

Proc. Soc. Exp. Biol. Med. 122:783-786(1966)

PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Exp. Cell Biol. 52:361-370(1984)

PubMed=2228906; DOI=10.1007/BF02624618

Niederkorn J.Y., Meyer D.R., Ubelaker J.E., Martin J.H.

Ultrastructural and immunohistological characterization of the SIRC corneal cell line.

In Vitro Cell. Dev. Biol. 26:923-930(1990)

PubMed=29653143; DOI=10.1016/j.exer.2018.04.004

Olivieri M., Cristaldi M., Pezzino S., Rusciano D., Tomasello B., Anfuso C.D., Lupo G.

Phenotypic characterization of the SIRC (Statens Seruminstitut Rabbit Cornea) cell line reveals a mixed epithelial and fibroblastic nature.

Exp. Eye Res. 172:123-127(2018)