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人肝癌細胞SK-HEP-1

人肝癌細胞SK-HEP-1

簡要描述:青旗(上海)生物技術發展有限公司,總部位于上海浦東新區,依托本地高校資源,逐步發展成為以生物技術為主的研發、生產、培訓為一體的綜合化產業平臺,在標準化細胞庫建立及細胞藥物前端模型方面成果顯著。公司生產經營原代細胞、細胞系、ELISA試劑盒、感受態細胞和HPLC檢測等科研產品與服務。我們秉承對用戶負責的態度,以對科研的高度嚴謹,以嚴格的質量控制,為廣大生物醫學科研用戶提供更優質的服務!

更新時間:2021-05-25

廠商性質:生產廠家

瀏覽次數:369

詳情介紹
品牌其他品牌貨號BFN60800688
規格T25培養瓶x1 1.5ml凍存管x2供貨周期現貨
主要用途僅供科研應用領域醫療衛生,生物產業

細胞名稱

人肝癌細SK-HEP-1                  

img1

貨物編碼

BFN60800688

產品規格

T25培養x1

1.5ml凍存x2

細胞數量

1x10^6

1x10^6

保存溫度

37

-198

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研用途

 

培養體系

DMEM高糖培養基Hyclone+10%胎牛血清Gibco+1%雙抗Hyclone

培養溫度

37

二氧化碳濃度

5%

簡介

人肝癌細胞SK-HEP-1細胞已被鑒定為內皮來源。該細胞系為異倍體女性人XX),染色體在亞三倍體范圍內。在裸鼠中,它能形成與肝癌相一致的大細胞癌。人肝癌細胞SK-HEP-1細胞由青旗(上海)生物技術發展有限公司2017年引種ATCC(HTB-52)

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

From: Memorial Sloan Kettering Cancer Center; New York; USA.

Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-535.

Characteristics: Has lost chromosome Y.

Doubling time: 46.7 +- 10.3 hours, 94.2 hours (in CDM4-CHO medium), 289.8 hours (in 293 SFM II medium) (PubMed=25822314); ~30 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Secretome proteome analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

AmelogeninX,X;CSF1PO11,12;D12S39118,18D13S3178,12;D16S53912,12;D18S5113,15;D19S43312,15.2D21S1129,31;D2S133820,23;D3S135816,16D5S81810,13;D6S104311,11;D7S8208,11;D8S117913,14FGA17,17;PentaE13,21;TH017,9;TPOX9,9;vWA14,17

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驗收細胞注意事 

1、收到人肝癌細SK-HEP-1細胞,請查看瓶子是否有破裂,培養基是否漏出,是否渾濁,如有請盡快聯系 

2、收到人肝癌細SK-HEP-1細胞,如包裝完好,請在顯微鏡下觀察細胞。,由于運輸過程中的問題,細胞培養瓶中的貼壁細胞有可能從瓶壁中脫落下來,顯微鏡下觀察會出現細胞懸浮的情況,出現此狀態時,請不要打開細胞培養瓶,應立即將培養瓶置于細胞培養箱里靜 3-5 小時左右,讓細胞先穩定下,再于顯微鏡下觀察,此時多數細胞會重新貼附于瓶壁。如細胞仍不能貼壁,請用臺盼藍染色法鑒定細胞活力,如臺盼藍染色證實細胞活力正常請按懸浮細胞的方法處理 

3、收到人肝癌細SK-HEP-1細胞后,請鏡下觀察細胞,用恰當方式處理細胞。若懸浮的細胞較多,請離心收集細胞,接種到一個新的培養瓶中。棄掉原液,使用新鮮配制的培養基,使用進口胎牛血清。剛接到細胞,若細胞不多 血清濃度可以加 15%去培養。若細胞迏 80% ,血清濃度還是 10 

4、收到人肝癌細SK-HEP-1細胞時如無異常情 ,請在顯微鏡下觀察細胞密度,如為貼壁細胞,未超80%匯合度時,將培養瓶中培養基吸出,留 5-10ML 培養基繼續培養:超 80%匯合度時,請按細胞培養條件傳代培養。如為懸浮細胞,吸出培養液,1000 /分鐘離 3 分鐘,吸出上清,管底細胞用新鮮培養基懸浮細胞后移回培養瓶。 

5、將培養瓶置 37培養箱中培養,蓋子微微擰松。吸出的培養基可以保存在滅菌過的瓶子里,存放 4冰箱,以備不時之需 

6、24 小時后,人肝癌細SK-HEP-1細胞形態已恢復并貼滿瓶壁,即可傳代。(貼壁細胞)將培養瓶里的培養基倒去, 3-5ml(以能覆蓋細胞生長面為準PBS  Hanks液洗滌后棄去。 0.5-1ml 0.25% EDTA 的胰酶消化,消化時間以具體細胞為準,一 1-3 分鐘,不超 5 分鐘??梢苑?/span>37培養箱消化。輕輕晃動瓶壁,見細胞脫落下來,加 3-5ml 培養基終止消化。用移液管輕輕吹打瓶壁上的細胞,使之*脫落,然后將溶液吸入離心管內離心1000rpm/5min。棄上清,視細胞數量決定分瓶數,一般一傳二,如細胞量多可一傳三,有些細胞不易傳得過稀,有些生長較快的細胞則可以多傳幾瓶,以具體細胞和經驗為準。(懸浮細胞)用移液管輕輕吹打瓶壁,直接將溶液吸入離心管離心即可 

7、貼壁細 ,懸浮細胞。嚴格無菌操作。換液時,換新的細胞培養瓶和換新鮮的培養液,375%CO2 培養。

 

特別提醒 原瓶中培養基不宜繼續使用,請更換新鮮培養基培養。



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