品牌 | 其他品牌 | 貨號 | BFN60810358 |
---|---|---|---|
規格 | T25培養瓶x1 1.5ml凍存管x2 | 供貨周期 | 現貨 |
主要用途 | 僅供科研 | 應用領域 | 醫療衛生,生物產業 |
細胞名稱 | 人B淋巴細胞GM12878 | ||
貨物編碼 | BFN60810358 | ||
產品規格 | T25培養瓶x1 | 1.5ml凍存管x2 | |
細胞數量 | 1x10^6 | 1x10^6 | |
保存溫度 | 37℃ | -198℃ | |
運輸方式 | 常溫保溫運輸 | 干冰運輸 | |
安全等級 | 1 | ||
用途限制 | 僅供科研 3類 |
培養體系 | 90%DMEM(不含丙酮酸鈉)+10%FBS+1%三抗 | ||
培養溫度 | 37℃ | 二氧化碳濃度 | 5% |
簡介 | 人B淋巴細胞GM12878取自女性外周血。 | ||
注釋 | Part of: CEPH/Utah pedigree cell line collection. Part of: ENCODE project common cell types; tier 1. Part of: Genetic Testing Reference Material (GeT-RM) samples. Part of: Genome in a Bottle (GIAB) consortium samples. Part of: International Genome Sample Resource (1000 genomes project) cell lines. Registration: CEPH Families Reference Panel; 146302. Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001. Population: Utah residents with ancestry from Northern and Western Europe. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). Omics: CNV analysis. Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: DNA methylation analysis. Omics: Deep RNAseq analysis. Omics: Genome sequenced. Omics: H3K27ac ChIP-seq epigenome analysis. Omics: H3K27me3 ChIP-seq epigenome analysis. Omics: H3K36me3 ChIP-seq epigenome analysis. Omics: H3K4me1 ChIP-seq epigenome analysis. Omics: H3K4me2 ChIP-seq epigenome analysis. Omics: H3K4me3 ChIP-seq epigenome analysis. Omics: H3K79me2 ChIP-seq epigenome analysis. Omics: H3K9ac ChIP-seq epigenome analysis. Omics: H3K9me3 ChIP-seq epigenome analysis. Omics: H4K20me1 ChIP-seq epigenome analysis. Omics: Mitochondrial genome sequenced. Omics: SNP array analysis. Omics: Transcriptome analysis. Derived from sampling site: Peripheral blood. | ||
基因突變 | CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C19*2 (Coriell). | ||
HLA信息 | / | ||
STR信息 | / | ||
參考文獻 | PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25 Morcos L., Ge B., Koka V., Lam K.C., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T. Genome-wide assessment of imprinted expression in human cells. Genome Biol. 12:R25.1-R25.14(2011)
PubMed=23325432; DOI=10.1101/gr.147942.112 Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M. Dynamic DNA methylation across diverse human cell lines and tissues. Genome Res. 23:555-567(2013)
PubMed=23676674; DOI=10.1038/nature12223 Wu L., Candille S.I., Choi Y., Xie D., Jiang L., Li-Pook-Than J., Tang H., Snyder M. Variation and genetic control of protein abundance in humans. Nature 499:79-82(2013)
PubMed=24185094; DOI=10.1038/nbt.2728 Selvaraj S., Dixon J.R., Bansal V., Ren B. Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing. Nat. Biotechnol. 31:1111-1118(2013)
PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005 Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.H., Toji L.H., Turner A., Kalman L.V. Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project. J. Mol. Diagn. 18:109-123(2016)
PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096 Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B. Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines. Cell Rep. 25:2617-2633(2018)
PubMed=31273215; DOI=10.1038/s41597-019-0116-4 Osorio D., Yu X., Yu P., Serpedin E., Cai J.J. Single-cell RNA sequencing of a European and an African lymphoblastoid cell line. Sci. Data 6:112-112(2019)
PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007 Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V. Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project. J. Mol. Diagn. 21:1034-1052(2019) |
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